ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of simulated navigation stimulation on the anesthetic sensitivity of sevoflurane in rats, so as to provide basis for rational using sevoflurane during navigation.</p><p><b>METHODS</b>SD rats were stimulated by Crampton model and the conditioned taste aversion (CTA) was regarded as criterion of motion sickness. (1) 60 rats were randomly divided into control (n = 15) and rotation group (n = 45). The changes of behavior and autonomic activity, sevoflurane concentration achieved sleep and anesthesia states, and the revitalization time were observed in two group rats. (2) 32 rats were randomly divided into control (I), rotation (II), anesthesia (III) and rotation plus anesthesia (IV) group (n = 8). The acetylcholine (Ach), norepinephrine (NE), r-aminobutyric acid (GABA), glutamic acid (Glu) of brain cortex, thalamus and hippocampus were determined respectively in the four group rats.</p><p><b>RESULTS</b>In control group, the sevoflurane concentration achieved sleep and anesthesia states were 1.74% +/- 0.05% and 3.54% +/- 0.05% respectively, but, those concentrations were 1.51% +/- 0.06% and 3.14% +/- 0.08% in rotation group. There were lower significantly in rotation group than those in control group (P < 0.01). It was a major characteristic that all of the neurotransmitters were reduced significantly in II group, this was even more in brain cortex and thalamus (P < 0.01). In II group, Ach was upward in brain cortex, NE and GABA were reduced in hippocampus obviously. The change tendency of neurotransmitters in IV group was more close to II group, that was, the effect of rotation stimulation was more obvious.</p><p><b>CONCLUSION</b>The anesthetic sensitivity of sevoflurane could be obvious increased in rats simulated navigation stimulation.</p>
Subject(s)
Animals , Male , Rats , Anesthetics , Pharmacology , Cerebral Cortex , Metabolism , Gravity, Altered , Hippocampus , Metabolism , Methyl Ethers , Pharmacology , Neurotransmitter Agents , Norepinephrine , Physical Conditioning, Animal , Rats, Sprague-Dawley , Rotation , Thalamus , Metabolism , gamma-Aminobutyric AcidABSTRACT
By reviewing five medical tangle cases after traumatic injury, the characters of these cases and the cause of medical malpractice had been discussed. After the analysis of the responsibility to the death caused by traumatic injury and medical malpractice, the assessment principle of cooperative competition of causes and degree of participation had been introduced. Meanwhile, it is believed that if comprehensive postmortem examination and pathologic examination were carried out to verify the death cause at first, they would play more important role in the appraisal of medical tangle cases after traumatic injury.